Results
PMID | 7843438 |
Gene Name | CCL2 |
Condition | Endometriosis |
Association |
Associated |
Population size | 12 |
Population details | 12 (6 women presenting for infertility or pelvic pain in which endometriosis was diagnosed at laparoscopy, 6 women presenting for tubal ligation without laparoscopic evidence of the disease) |
Sex | Female |
Infertility type | Female infertility |
Associated genes | MCP-1 |
Other associated phenotypes |
Endometriosis associated infertility, Infertility, pelvic pain |
Fertil Steril. 1995 Feb;63(2):322-8. Akoum, A| Lemay, A| Brunet, C| Hebert, J Universite Laval, Quebec, Canada. OBJECTIVE: To evaluate in vitro the production of monocyte chemotactic protein-1 (MCP-1) by endometrial cells of patients with and without endometriosis. DESIGN: Primary cultures of stromal and epithelial cells isolated from human endometrium were exposed during 24 hours to different cytokines. Monocyte chemotactic protein-1 secretion was analyzed in the culture medium. SETTING: Gynecology clinic and laboratories of endocrinology of reproduction and immunology. PATIENTS: Women presenting for infertility or pelvic pain in which endometriosis was diagnosed at laparoscopy (n = 6) and women presenting for tubal ligation without laparoscopic evidence of the disease (n = 6). INTERVENTIONS: None. MAIN OUTCOME MEASURES: De novo secretion of MCP-1 in the culture supernatant by immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis after metabolic labeling with 35S-cysteine. RESULTS: The incubation of endometrial epithelial cells of endometriosis women with either interleukin-1 beta or tumor necrosis factor-alpha resulted in the appearance of at least two and sometimes three bands having approximately 15, 13, and 9 kd molecular weights. These bands were identified as three distinct species of MCP-1 as their immunoprecipitation was prevented effectively in presence of an excess of cold MCP-1. In contrast, the endometrial epithelial cells of only one of six normal women produce significant levels of MCP-1 under the same stimulation conditions. The stromal cells of both groups of subjects do not secrete appreciable amounts of MCP-1 or only small quantities in two cases of endometriosis. CONCLUSIONS: Monocyte chemotactic protein-1 secretion is upregulated in cytokine-stimulated endometrial epithelial cells of women having endometriosis as compared with normal women without evidence of the disease. Such a difference at the level of eutopic endometrial cell may have a significance in the physiopathology of endometriosis. Mesh Terms: Adult| Cells, Cultured| Chemokine CCL2| Chemotactic Factors/biosynthesis/*secretion| Endometriosis/pathology/*physiopathology| Endometrium/pathology/*secretion| Epithelium/pathology| Female| Humans| Immunohistochemistry| Immunosorbent Techniques |